100-0688
STMC100-0688EA
892
EUR
InStock
STMC100-0688
Mouse ACE2 ELISA Kit
Tests
Tests ELISA
For detection and measurement of mouse ACE2.
The Mouse ACE2 ELISA Kit is designed for the quantitative detection and measurement of mouse angiotensin converting enzyme 2 (ACE2) in biological fluids such as serum, plasma, cell culture supernatant, and tissue homogenate. ACE2 is a type I integral membrane protein. It is highly expressed in capillary-rich organs such as the lungs, heart, blood vessels, kidneys, liver, and gastrointestinal tract.
The ACE2 receptor is the target of the SARS-CoV-2 Spike (S) glycoprotein. This interaction allows for viral entry into the host cell, making ACE2 a potential target for antiviral therapeutics. The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for ACE2.
The captured ACE2 is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’, 5,5’ tetramethylbenzidine (TMB) results in a coloured product with an intensity directly proportional to the concentration of ACE2 in the sample.
The concentration of ACE2 is determined by comparison to a serial dilution of the standard analysed in parallel.
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