0981-400MLEA
282
EUR
InStock
0981-400ML
Phénol ≥99.0% pour les biotechnologies pH 4.5
Phénol
An ideal replacement for water-saturated phenol in many protocols. Offers the high purity and performance of water saturated phenol with greatly enhanced stability and safety.
- Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
- Liquid phenols offer a safer, easier alternative to crystalline phenol
- Optimised pH for the purification of RNA
- Long term stability > one year
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation.