30126
30126.EA
507
EUR
InStock
30126.
ExoBrite™ WGA EV Staining Kits
Tests
Tests cellulaires
ExoBrite™ WGA EV staining kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV stains bind to molecules in the EV membrane for bright, specific staining, with little background.
- Optimally formulated WGA conjugates for staining purified EVs
- Broad compatibility, stained EVs isolated from all 9 sources tested
- Designed for detection by flow cytometry
- Bright signal and low background
- Compatible with antibody co-staining
- Available in four colours
ExoBrite™ WGA EV stains are uniquely formulated conjugates of wheat germ agglutinin (WGA), a carbohydrate-binding lectin with high affinity for N-acetylglucosamine moieties of glycoproteins. WGA conjugates are often used for labeling cell membranes as well as gram-positive bacteria. WGA conjugates have also been used to detect EVs due to the presence of glycoproteins on EV membranes.
ExoBrite™ WGA EV stains were designed to overcome some of the challenges of detecting isolated EVs, particularly in flow cytometry. For example, tetraspanin antibodies commonly used to stain EVs can have varying signal and coverage depending on the EV source. Conversely, ExoBrite™ WGA EV stains show bright staining of EVs derived from a broad range of sources. We tested EVs derived from 9 cell lines and ExoBrite™ WGA EV stains showed strong staining for all of them. ExoBrite™ WGA EV stains are less prone to aggregation than hydrophobic membrane dyes and do not bind non-specifically to polystyrene beads, allowing them to be used to stain bead-bound EVs.
EVs are often labeled with fluorescent antibodies targeting one or more of the tetraspanin proteins CD9, CD63, and CD81. ExoBrite™ WGA staining can be combined with antibody staining, for multi-parameter analysis.
Kit components
- ExoBrite™ WGA EV stain
- ExoBrite™ 1X PBS solution