40014
40014.EA
396
EUR
InStock
40014.
Ethidium homodimer I 2 mM dans le DMSO utilisé en coloration nucléaire
Ethidium homodimer I
Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.
- λEx/λEm (with DNA) = 528/617 nm
- λAbs (in H2O, no DNA) = 493 nm
- Cell permeability: Membrane impermeant
- Colors: Red
- Red solid soluble in DMSO or MeOH
- Store at 4°C and protect from light
Biotium offers a high purity grade Ethidium Homodimer I that is not available from other manufacturers. Ethidium Homodimer from other suppliers often contains a high amount (as much as 20%) of inorganic salt that lowers the weight percent purity. Biotium also offers Ethidium Homodimer I in solid form (catalog no. 40010).
Also see Ethidium Homodimer III, which was developed by Biotium as a superior alternative to Ethidium Homodimer I. The absorption and emission spectra are similar, but EthD-III is 45% brighter.
EthD-I is dead cell specific in all cell types, including mammalian cells, bacteria and yeast.