40047
40047.EA
313
EUR
InStock
40047.
bisBenzimide H-33342 trihydrochloride trihydrate (Hoechst 33342)
bisBenzimide H-33342 trihydrochloride trihydrate (Hoechst 33342)
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains.
- λEx/λEm = 350/461 nm (with DNA)
- Pale yellow solid soluble in water up to 2% (w/v)
- Store at 4°C and protect from light
Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258. Either dye can be used to stain live or fixed cells in buffer or medium at 1 ug/mL with no wash step. Biotium also offers Hoechst 33342, 10 mg/mL in H2O (catalog no. 40046).
Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.