70014
70014.EA
364
EUR
InStock
70014.
JC-1 iodide salt colorant pour mitochondries, convient pour la fluorescence
JC-1 iodide salt
JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine, iodide) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.
- Cellular localization: Mitochondria
- For live or fixed cells: For live/intact cells
- Assay type/options: No-wash staining, Real-time imaging
- Cell permeability: Membrane permeant
- Apoptosis/viability marker: Mitochondrial potential
- Potential dependence: Mitochondrial potential-dependent
- Indicator type: Ratiometric
- Colors: Green/Red
- Excitation/Emission: 510/527 nm (monomer); 585/590 nm (aggregate)
- Red solid soluble in DMSO
- Store at 4°C and protect from light, especially in solution
JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine, iodide) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (λ Em = 527 nm), while the J-aggregates have red fluorescence (λ Em = 590 nm). Therefore, it is possible to use fluorescence ratioing technique to study mitochondrial membrane potentials. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green. It has also been used in high throughput drug screening applications.
Biotium offers JC-1 as either iodide salt or chloride salt (#70011). Both forms have identical spectral properties. The iodide salt is the more commonly used form, however some researchers may prefer the chloride salt because chloride is the most prevalent anion in biological systems.