A302555-96
ANTIA302555-96EA
685
EUR
InStock
ANTIA302555-96
Canine Angiotensin ll ELISA Kit
Tests
Tests ELISA
Canine Angiotensin ll ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of canine Angiotensin ll in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Canine Angiotensin ll ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of canine Angiotensin ll in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Angiotensin ll antigen. During the incubation, Angiotensin ll present in the samples or standards competes with the fixed amount of immobilised Angiotensin ll for binding sites on the Biotinylated anti-Angiotensin ll antibody. The more Angiotensin ll present in a sample or standard, the less Biotinylated anti-Angiotensin ll antibody that binds to the plate. Following incubation, unbound Biotinylated anti-Angiotensin ll antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Angiotensin ll present in each sample or standard. The concentration of Angiotensin ll can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.