A303047-96
ANTIA303047-96EA
610
EUR
InStock
ANTIA303047-96
Human GluN2D ELISA Kit
Tests
Tests ELISA
Human GluN2D ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human GluN2D in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 31,25 to 2000 pg/ml
- Sensitivity: 18,75 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Human GluN2D ELISA kit (A303047) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human GluN2D in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for GluN2D has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GluN2D present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-GluN2D antibody, which binds the captured GluN2D present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of GluN2D captured in each well. The concentration of GluN2D can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready to use ELISA kit that contains: pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.