A303891-96
ANTIA303891-96EA
630
EUR
InStock
ANTIA303891-96
Heparan Sulfate ELISA Kit
Tests
Tests ELISA
Heparan Sulfate ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Heparan Sulfate in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 78 to 5000 ng/ml
- Sensitivity: 46,9 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Heparan Sulfate ELISA kit (A303891) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Heparan Sulfate in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Heparan Sulfate antigen. During the incubation, Heparan Sulfate present in the samples or standards competes with the fixed amount of immobilised Heparan Sulfate for binding sites on the Biotinylated Anti-Heparan Sulfate antibody. The more Heparan Sulfate present in a sample or standard, the less Biotinylated Anti-Heparan Sulfate antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Heparan Sulfate antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Heparan Sulfate present in each sample or standard. The concentration of Heparan Sulfate can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready to use ELISA kit that contains: pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.