A310150-96
ANTIA310150-96EA
525
EUR
InStock
ANTIA310150-96
Human Midkine ELISA Kit
Tests
Tests ELISA
Human Midkine ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Midkine in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 75 to 1200 pg/ml
- Sensitivity: 2,49 pg/ml
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human Midkine ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Midkine in serum, plasma or other biological fluids. An antibody specific for Midkine has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Midkine present in each sample is bound to the wells by the immobilised antibody. Biotinylated Anti-Midkine antibody, which also binds the Midkine present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Midkine antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Midkine antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Midkine captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Midkine in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, streptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.