A310166-96
ANTIA310166-96EA
525
EUR
InStock
ANTIA310166-96
Human ITIH1/SHAP ELISA Kit
Tests
Tests ELISA
Human ITIH1/SHAP ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human ITIH1/SHAP in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 80 to 1280 ng/ml
- Sensitivity: 6,19 ng/ml
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human ITIH1/SHAP ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human ITIH1/SHAP in serum, plasma or other biological fluids. An antibody specific for ITIH1/SHAP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the ITIH1/SHAP present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-ITIH1/SHAP antibody, which also binds the ITIH1/SHAP present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-ITIH1/SHAP antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-ITIH1/SHAP antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of ITIH1/SHAP captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of ITIH1/SHAP in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, streptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.