A310205-96
ANTIA310205-96EA
545
EUR
InStock
ANTIA310205-96
Human PHD2/Prolyl Hydroxylase ELISA Kit
Tests
Tests ELISA
Human PHD2/Prolyl Hydroxylase ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human PHD2/Prolyl Hydroxylase in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 150 to 2400 ng/L
- Sensitivity: 10,14 ng/L
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human PHD2/Prolyl Hydroxylase ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human PHD2/Prolyl Hydroxylase in serum, plasma or other biological fluids. An antibody specific for PHD2/Prolyl Hydroxylase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PHD2/Prolyl Hydroxylase present in each sample is bound to the wells by the immobilised antibody. Biotinylated Anti-PHD2/Prolyl Hydroxylase antibody, which also binds the PHD2/Prolyl Hydroxylase present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-PHD2/Prolyl Hydroxylase antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-PHD2/Prolyl Hydroxylase antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of PHD2/Prolyl Hydroxylase captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of PHD2/Prolyl Hydroxylase in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, streptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.