A310315-96
ANTIA310315-96EA
545
EUR
InStock
ANTIA310315-96
Human SP17 ELISA Kit
Tests
Tests ELISA
Human SP17 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human SP17 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 150 to 2400 ng/L
- Sensitivity: 10,16 ng/L
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human SP17 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human SP17 in An antibody specific for SP17 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the SP17 present in each sample is bound to the wells by the immobilised antibody. Biotinylated Anti-SP17 antibody, which also binds the SP17 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-SP17 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-SP17 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of SP17 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of SP17 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, streptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.