A311039-96
ANTIA311039-96EA
545
EUR
InStock
ANTIA311039-96
Human G-CSF ELISA Kit
Tests
Tests ELISA
Human G-CSF ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human G-CSF in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 1,5 to 24 ng/ml
- Sensitivity: 0,053 ng/ml
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human G-CSF ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human G-CSF in serum, plasma or other biological fluids. An antibody specific for G-CSF has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the G-CSF present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-G-CSF antibody, which also binds the G-CSF present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-G-CSF antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-G-CSF antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of G-CSF captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of G-CSF in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, streptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.