A312431-96
ANTIA312431-96EA
545
EUR
InStock
ANTIA312431-96
Human SYT14 ELISA Kit
Tests
Tests ELISA
Human SYT14 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human SYT14 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 62,5 to 1000 ng/L
- Sensitivity: 3,15 ng/L
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human SYT14 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human SYT14 in An antibody specific for SYT14 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the SYT14 present in each sample is bound to the wells by the immobilised antibody. Biotinylated Anti-SYT14 antibody, which also binds the SYT14 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-SYT14 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-SYT14 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of SYT14 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of SYT14 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, streptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.