A312758-96
ANTIA312758-96EA
545
EUR
InStock
ANTIA312758-96
Human EPF ELISA Kit
Tests
Tests ELISA
Human EPF ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human EPF in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection range: 0,75 to 12 ng/ml
- Sensitivity: 0,026 ng/ml
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human EPF ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human EPF in serum, plasma or other biological fluids. An antibody specific for EPF has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the EPF present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-EPF antibody, which also binds the EPF present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-EPF antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-EPF antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of EPF captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of EPF in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, steptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.