A312914-96
ANTIA312914-96EA
525
EUR
InStock
ANTIA312914-96
Mouse Adenosine A1 Receptor ELISA Kit
Tests
Tests ELISA
Mouse Adenosine A1 receptor ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Adenosine A1 receptor in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Mouse Adenosine A1 receptor ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Adenosine A1 receptor in serum, plasma or other biological fluids. An antibody specific for Adenosine A1 receptor has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Adenosine A1 receptor present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-Adenosine A1 receptor antibody, which also binds the Adenosine A1 receptor present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Adenosine A1 receptor antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Adenosine A1 receptor antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Adenosine A1 receptor captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Adenosine A1 receptor in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, positive control, negative control, HRP-conjugated detection antibody, sample diluent, substrate solution A, substrate solution B, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.