A74229-96
ANTIA74229-96EA
525
EUR
InStock
ANTIA74229-96
Noradrenaline/Norepinephrine ELISA Kit
Tests
Tests ELISA
Noradrenaline/Norepinephrine ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Noradrenaline/Norepinephrine in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection Range: 15,625 to 1000 pg/ml
- Sensitivity: 9,375 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Noradrenaline/Norepinephrine ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Noradrenaline/Norepinephrine in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Noradrenaline/Norepinephrine antigen. During the incubation, Noradrenaline/Norepinephrine present in the samples or standards competes with the fixed amount of immobilised Noradrenaline/Norepinephrine for binding sites on the Biotinylated Anti-Noradrenaline/Norepinephrine Antibody. The more Noradrenaline/Norepinephrine present in a sample or standard, the less Biotinylated Anti-Noradrenaline/Norepinephrine Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Noradrenaline/Norepinephrine Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Noradrenaline/Norepinephrine present in each sample or standard. The concentration of Noradrenaline/Norepinephrine can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.