A74358-96
ANTIA74358-96EA
600
EUR
InStock
ANTIA74358-96
Human C9orf140 ELISA Kit
Tests
Tests ELISA
Human C9orf140 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human C9orf140 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection Range: 0,781 to 50 ng/ml
- Sensitivity: 0,469 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human C9orf140 ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of human C9orf140 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with C9orf140 antigen. During the incubation, C9orf140 present in the samples or standards competes with the fixed amount of immobilised C9orf140 for binding sites on the Biotinylated Anti-C9orf140 Antibody. The more C9orf140 present in a sample or standard, the less Biotinylated Anti-C9orf140 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-C9orf140 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of C9orf140 present in each sample or standard. The concentration of C9orf140 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.