A74391-96
ANTIA74391-96EA
545
EUR
InStock
ANTIA74391-96
Free Estriol ELISA Kit
Tests
Tests ELISA
Free Estriol ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Free Estriol in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection range: 0,625 to 40 ng/ml
- Sensitivity: 0,375 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Free Estriol ELISA kit (A74391) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Free Estriol in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Free Estriol antigen. During the incubation, Free Estriol present in the samples or standards competes with the fixed amount of immobilised Free Estriol for binding sites on the Biotinylated Anti-Free Estriol Antibody. The more Free Estriol present in a sample or standard, the less Biotinylated Anti-Free Estriol Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Free Estriol Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Free Estriol present in each sample or standard. The concentration of Free Estriol can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin Conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.