A74786-96
ANTIA74786-96EA
600
EUR
InStock
ANTIA74786-96
Human FAM214A ELISA Kit
Tests
Tests ELISA
Human FAM214A ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human FAM214A in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Detection range: 0,781 to 50 ng/ml
- Sensitivity: 0,469 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human FAM214A ELISA kit (A74786) employs the competitive enzyme immunoassay technique for the quantitative measurement of human FAM214A in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with FAM214A antigen. During the incubation, FAM214A present in the samples or standards competes with the fixed amount of immobilised FAM214A for binding sites on the Biotinylated Anti-FAM214A Antibody. The more FAM214A present in a sample or standard, the less Biotinylated Anti-FAM214A Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-FAM214A Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of FAM214A present in each sample or standard. The concentration of FAM214A can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin Conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.