A77504-96
ANTIA77504-96EA
600
EUR
InStock
ANTIA77504-96
Human Wnt2b ELISA Kit
Tests
Tests ELISA
Human Wnt2b ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Wnt2b in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 15,625 to 1000 pg/ml
- Sensitivity: 9,375 pg/ml
- Sample type: Serum, plasma, tissue homogenates and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Human Wnt2b employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Wnt2b in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Wnt2b has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Wnt2b present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Wnt2b Antibody, which binds the captured Wnt2b present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Wnt2b captured in each well. The concentration of Wnt2b can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.