A77637-96
ANTIA77637-96EA
580
EUR
InStock
ANTIA77637-96
Human ADAL ELISA Kit
Tests
Tests ELISA
Human ADAL ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human ADAL in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 0,313 to 20 ng/ml
- Sensitivity: 0,188 ng/ml
- Sample type: Serum, plasma, tissue homogenates and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Human ADAL employs the competitive enzyme immunoassay technique for the quantitative measurement of human ADAL in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with ADAL antigen. During the incubation, ADAL present in the samples or standards competes with the fixed amount of immobilised ADAL for binding sites on the Biotinylated Anti-ADAL Antibody. The more ADAL present in a sample or standard, the less Biotinylated Anti-ADAL Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-ADAL Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of ADAL present in each sample or standard. The concentration of ADAL can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.