A78120-96
ANTIA78120-96EA
580
EUR
InStock
ANTIA78120-96
Mouse G-6-Pase Catalytic Subunit 1 ELISA Kit
Tests
Tests ELISA
Mouse G-6-Pase Catalytic Subunit 1 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse G-6-Pase Catalytic Subunit 1 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 0,781 to 50 ng/ml
- Sensitivity: 0,469 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse G-6-Pase Catalytic Subunit 1 ELISA kit (A78120) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse G-6-Pase Catalytic Subunit 1 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for G-6-Pase Catalytic Subunit 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the G-6-Pase Catalytic Subunit 1 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-G-6-Pase Catalytic Subunit 1 Antibody, which binds the captured G-6-Pase Catalytic Subunit 1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of G-6-Pase Catalytic Subunit 1 captured in each well. The concentration of G-6-Pase Catalytic Subunit 1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.