A79849-96
ANTIA79849-96EA
580
EUR
InStock
ANTIA79849-96
Human Malondialdehyde ELISA Kit
Tests
Tests ELISA
Human Malondialdehyde ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human Malondialdehyde in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA Kit
- Detection range: 7,813 to 500 ng/ml
- Sensitivity: 4,688 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human Malondialdehyde ELISA Kit employs the competitive enzyme immunoassay technique for the quantitative measurement of human Malondialdehyde in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Malondialdehyde antigen. During the incubation, Malondialdehyde present in the samples or standards competes with the fixed amount of immobilised Malondialdehyde for binding sites on the Biotinylated Anti-Malondialdehyde Antibody. The more Malondialdehyde present in a sample or standard, the less Biotinylated Anti-Malondialdehyde Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Malondialdehyde Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Malondialdehyde present in each sample or standard. The concentration of Malondialdehyde can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.