A79857-96
ANTIA79857-96EA
580
EUR
InStock
ANTIA79857-96
Rat Prostaglandin E2 ELISA Kit
Tests
Tests ELISA
Rat Prostaglandin E2 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat Prostaglandin E2 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA Kit
- Detection range: 31,25 to 2000 pg/ml
- Sensitivity: 18,75 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rat Prostaglandin E2 ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of rat Prostaglandin E2 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Prostaglandin E2 antigen. During the incubation, Prostaglandin E2 present in the samples or standards competes with the fixed amount of immobilised Prostaglandin E2 for binding sites on the Biotinylated Anti-Prostaglandin E2 Antibody. The more Prostaglandin E2 present in a sample or standard, the less Biotinylated Anti-Prostaglandin E2 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Prostaglandin E2 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Prostaglandin E2 present in each sample or standard. The concentration of Prostaglandin E2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin Conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.