A86980-96
ANTIA86980-96EA
580
EUR
InStock
ANTIA86980-96
Human alpha Endorphin ELISA Kit
Tests
Tests ELISA
Human alpha Endorphin ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human alpha Endorphin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA Kit
- Detection range: 15,625 to 1000 pg/ml
- Sensitivity: 9,375 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human alpha Endorphin ELISA Kit employs the competitive enzyme immunoassay technique for the quantitative measurement of human alpha Endorphin in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with alpha Endorphin antigen. During the incubation, alpha Endorphin present in the samples or standards competes with the fixed amount of immobilised alpha Endorphin for binding sites on the Biotinylated Anti-alpha Endorphin Antibody. The more alpha Endorphin present in a sample or standard, the less Biotinylated Anti-alpha Endorphin Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-alpha Endorphin Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of alpha Endorphin present in each sample or standard. The concentration of alpha Endorphin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.