A87460-96
ANTIA87460-96EA
600
EUR
InStock
ANTIA87460-96
Mouse Kynurenine ELISA Kit
Tests
Tests ELISA
Mouse Kynurenine ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse Kynurenine in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA Kit
- Detection range: 7,813 to 500 pmol/ml
- Sensitivity: 4,688 pmol/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Mouse Kynurenine ELISA kit employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse Kynurenine in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Kynurenine antigen. During the incubation, Kynurenine present in the samples or standards competes with the fixed amount of immobilised Kynurenine for binding sites on the Biotinylated Anti-Kynurenine Antibody. The more Kynurenine present in a sample or standard, the less Biotinylated Anti-Kynurenine Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Kynurenine Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Kynurenine present in each sample or standard. The concentration of Kynurenine can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Informations de commande: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin Conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.