252908.1608
APPC252908.1608EA
50.1
EUR
InStock
APPC252908.1608
Réactif Indole selon Kovac pour la microbiologie
Réactif Indole selon Kovac
A reagent for the detection of indole-positive and indole-negative microorganisms. Some microorganisms can cleave tryptophan which is especially abundant in trypticalle digested peptone to give pyruvic acid, ammonia and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a colour reaction with 4-dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
The strain purity of the organism to be tested must first be established; it is then inoculated into an appropriate culture medium and incubated for 18-24 hours at the optimal incubation temperature. The medium is then covered with a layer of KOVÁCS’ indole reagent of about 0,5 cm. If indole is present the reagent layer turns cherry red in colour after a few minutes.
Informations de commande: Contains 50 g 4-(Dimethylamino) benzaldehyde, 250 ml hydrochloric acid 37 % and 750 ml 1-butanol.