600590-96
CAYM600590-96EA
844
EUR
InStock
CAYM600590-96
Nrf2 transcription factor assay kit
Tests
Tests cellulaires
Nrf2 Transcription Factor Assay kit is a 96-well assay for measurement of Nrf2.
- Sensitive, non radioactive method of detecting Nrf2 from whole cell lysates
- 96-well plate format replaces EMSAs
- Capture the transcription factor using a specific dsDNA sequence bound to the plate
- Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
Nuclear factor E₂-related factor 2 (Nrf2) is a transcription factor that plays a key role in maintaining redox homeostasis via its interaction with a cysteine-rich protein kelch-like ECH-associated protein 1 (Keap1). In resting cells, Nrf2 and keap1 form a tight complex, which is targeted for degradation by proteasomes.
Under oxidative stress, Nrf2 is released from the Nrf2/keap1 complex and translocates to the nucleus where it is able to induce the expression of a battery of genes encoding diverse cytoprotective proteins, including antioxidative enzymes, anti-inflammatory mediators, and proteasomes. Nrf2 transcription factor assay is a non-radioactive, colorimetric method for detecting specific transcription factor DNA binding activity in nuclear extracts.
A specific double stranded DNA (dsDNA) sequence containing the Nrf2 response element is immobilised onto the wells of a 96-well plate. Nrf2 contained in nuclear extract samples will bind specifically to the Nrf2 response element and can be detected by addition of a specific Nrf2 antibody and a secondary antibody conjugated to HRP.
Information de livraison: Kit contains assay buffer, reagent A, positive control, binding buffer, antibody, concentrate, polysorbate 20, competitor dsDNA, GRP conjugate, plate and cover, seveloping and stop solution.