E277-400MLEA
195
EUR
InStock
E277-400ML
Phénol : Chloroforme : Alcool isoamylique (125:24:1) (v/v/v) ≥99.0%, Qualité Biotechnologie
Phénol : Chloroforme : Alcool isoamylique
Phenol Chloroform 5:1 Solution. An optimised mixture that can be used directly to replace the phenol and chloroform recommended in the one-step phenol purification method.
- Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
- Liquid phenols offer a safer, easier alternative to crystalline phenol
- Optimized pH for the Purification of RNA
- Long term stability > one year
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.