Synonyms:
H2DCFDA, 2,7-Dichlorodihydrofluorescein diacetate, DCDHF diacetate
ALX610022M050
ENZOALX610022M050EA
69.3
EUR
InStock
ENZOALX610022M050
2',7'-Dichlorofluorescin diacetate ≥95% (par HPLC)
2',7'-Dichlorofluorescin diacetate
Peroxynitrite indicator.
Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalysed cleavage of the diacetate groups, DCDHF is oxidised by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502 nm, Em(max): 523 nm), or by absorbance spectroscopy at 500 nm (ε = 59, 500 M⁻¹cm⁻¹). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidise DCDHF.
25 mg/ml soluble in DMSO, 100% ethanol or dimethyl formamide, sparingly soluble in aqueous buffers.