ENZ52054
ENZOENZ52054EA
512
EUR
InStock
ENZOENZ52054
Coelenterazine ≥90% (par HPLC)
Coelenterazine
Calcium dye.
Colenterazine is useful for monitoring calcium in live cells and tissues, in reporter gene assays, for superoxide anion detection and in drug high-throughput screening applications. Coelenterazine is useful for reconstituting aequorin in cells that have been transfected with apo-aequorin cDNA. The aequorin complex comprises a 22 kDa apoaequorin protein, molecular oxygen and coelenterazine. In the presence of Ca(II), coelenterazine is oxidized to coelenteramide, yielding the release of carbon dioxide and blue light. Unlike fluorescent Ca(II) indicators, Ca²⁺-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Wavelength Maxima: Excitation 429 nm, Emission 466 nm
Soluble in 100% ethanol.