Synonyms:
Folin & Ciocalteu’s phenol reagent
0219518690
ICNA0219518690EA
141
EUR
InStock
ICNA0219518690
Réactif de Folin-Ciocalteu pour le dosage des phénols
Réactif de Folin-Ciocalteu
Folin & Ciocalteu`s Phenol Reagent is suitable for determination of total protein by Lowry method. It is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric assay of phenolic and polyphenolic antioxidants.
- 2N Concentration
- Used in the Lowry method for determining protein concentration
- Spray reagent in chromatographic procedures
A procedure for preparation of the Folin & Ciocalteu's phenol reagent has been published. Dissolve 10 g of sodium tungstate and 2.5 g of sodium molybdate in 70 ml of water. Add 5 ml of 85% phosphoric acid and 10 ml of concentrated hydrochloric acid. Reflux for 10 hours. Add 15 g of lithium sulfate, 5 ml of water, and 1 drop of bromine. Reflux for 15 minutes. Cool to room temperature and bring to 100 ml with water.