21565
PIER21565EA
252
EUR
InStock
PIER21565
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)], Pierce™
EGS [Ethylene glycolbis(succinic acid-N-hydroxysuccinimide ester)]
Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
- Reactive groups: NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
- Membrane-permeable, allowing for intracellular crosslinking
- Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
- Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS
Attention: For Research Use Only. Not for use in diagnostic procedures.