24580
PIER24580EA
239
EUR
InStock
PIER24580
Pierce™ Reversible Protein Stain Kit for Nitrocellulose Membranes
Colorants
Colorants pour gels d'electrophorèse
The Pierce reversible protein stain kit for nitrocellulose membranes is a rapid and sensitive alternative to ponceau S stain for protein detection on nitrocellulose membranes after transfer from polyacrylamide gels.
- Sensitive high-avidity, total-protein stain lower limit of detection equals 25 to 50 ng per band
- Specific detects only protein does not bind or interact with other electrophoresis or sample components
- Protocol stain in less than five minutes completely erase and reverse staining in less than 15 minutes
- Stability components are stable at room temperature, saving refrigerator space and eliminating equilibration steps
This kit for membrane staining uses a nondestructive, reversible, reliable, and sensitive method to stain and detect proteins on nitrocellulose membranes. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional ponceau S staining). The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents. The staining protocol is simple and quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.
Contents, sufficient for 10 nitrocellulose membranes (8×8 cm) from mini gels (SDS-PAGE). Reversible stain 250 ml, destain 2×500 ml, stain eraser 250 ml.
Detection location: In-blot detection
Detection method: Colorimetric
Label or dye: Proprietary mix
Target molecule: Protein
Attention: Store at room temperature.