44988
PIER44988EA
750
EUR
InStock
PIER44988
Pierce™ F(ab')2 Preparation Kit
Protéines purification
Protein Purification Kits
Pierce F(ab')2 preparation kit uses immobilised pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.
- Immobilised pepsin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
- Include all reagents needed to prepare and purify antibody fragments
- Fast spin format greatly reduces sample processing time
- Flexible protocols are included for multiple species and IgG subclasses, as well as sample size and concentration
- Efficient enhanced yield and sample purity
Suitable for human, rabbit, mouse and other species and subclasses of IgG except mouse IgG1. The antibody digestion reaction with pepsin agarose is performed in convenient disposable spin columns that allow efficient removal of the immobilised protease and maximum recovery of the IgG fragments. Also included in the kits are NAb Protein A spin columns and buffers to efficiently purify the resulting fragments. Protein A binds the Fc fragments and undigested IgG, allowing the pure F(ab')2 fragments to be recovered in the flow-through fraction. The kits also include Zeba desalting spin columns for preparing the IgG sample quickly without dilution instead of utilising time-consuming dialysis steps.
The kits use pepsin, a nonspecific endopeptidase, to enzymatically digest the Fc portion of whole IgG to yield the fragment known as F(ab')2. This fragment is composed of a pair of Fab' units connected by two disulfide bonds. Because the pepsin protease is supplied in immobilised form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free.
Store at 4 to 8 °C.
Information de livraison: Kit includes Pierce Pepsin agarose (1,25 ml), F(ab')2 digestion buffer (120 ml), NAb Protein A Plus Spin Column (1 ml, 1 column), PBS packs (each makes 500 ml) (2 packs), IgG Elution buffer (120 ml), Zeba desalt spin columns (7 K MWCO, 2 ml, 10 columns), Spin columns, 0,8 ml, 10 columns), Microcentrifuge tubes (2 ml, 30 tubes).