A39269
PIERA39269EA
178
EUR
InStock
PIERA39269
Sulpho-NHS (N-Hydroxysulphosuccinimide sodium salt), No-Weigh™ Format, Pierce™
Sulpho-NHS (N-Hydroxysulphosuccinimide sodium salt)
Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
- Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
- Amine-reactive NHS esters or Sulfo-NHS esters can be made with any carboxyl-containing molecule
- Sulfo-NHS derivatives are usually directly water-soluble (can be added directly to physiologic buffers) and membrane-impermable (can be used for cell surface labeling)
- High-purity, crystalline Sulfo-NHS can be used to create high-quality activated derivatives
- Purity—Greater than 95% by quantitative NMR (the highest standard for crosslinker purity); average lot purity is greater than 99%
Compared to the standard grade product, Premium Grade Sulfo-NHS provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.
Attention: For Research Use Only. Not for use in diagnostic procedures.