Synonyms:
TNBT (tetra nitro-blue tetrazolium chloride), BCIP (5-bromo-4-chloro-3-indolylphosphate toluidine salt)
BCBT-100
ROCKBCBT-100EA
233
EUR
InStock
ROCKBCBT-100
BCIP/TNBT substrat de phosphatase alcaline
BCIP/TNBT
BCIP/TNBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. BCIP/TNBT can be utilized in ELISA, in situ hybridization, and IHC. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of TNBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
BCIP/TNBT Membrane Alkaline Phosphatase Substrate is a useful detection reagent for WB, IHC (immunohistochemistry), and in situ hybridisation.
Storage Condition: Store container at 4° C prior to opening. Protect BCIP/TNBT Membrane Alkaline Phosphatase Substrate from moisture and light.