03800
STMC03800EA
1406
EUR
InStock
STMC03800
ClonaCell™-HY Hybridoma Kit
Milieux de culture cellulaire
Complete kit for hybridoma generation.
Perform all the steps in hybridoma development and monoclonal antibody production using the media and reagents included in the ClonaCell™-HY Hybridoma Kit:
ClonaCell™-HY Medium A for myeloma and hybridoma culture
ClonaCell™-HY Medium B for hybridoma fusion
ClonaCell™-HY Medium C for hybridoma fusion recovery
ClonaCell™-HY Medium D for hybridoma selection and cloning
ClonaCell™-HY Medium E for hybridoma growth
ClonaCell™-HY PEG to support hybridoma fusion
The ClonaCell™-HY method uses a methylcellulose-based semi-solid selective medium to combine hybridoma selection and cloning into one step. Individual parental hybridoma clones and their progeny remain localised together in the semi-solid medium as they grow to form distinct colonies. This prevents the loss of rare clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium. The hybridoma colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion.
This kit has been verified for use in mouse and rat hydridoma development and monoclonal antibody production and reportedly is compatible for production, cloning, and expansion of hybridomas using lymphocytes from a variety of host animals including human, mouse, rat, and hamster. For your convenience, the kit components are also available for purchase individually.
Attention: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').