100-0505
STMC100-0505EA
605
EUR
InStock
STMC100-0505
CoV Protease Substrate-1 TF5
Protéines et Peptides
FRET-based peptide substrate for detection of protease activity of coronaviruses - 100 tests.
- Enables efficient detection of coronavirus protease activity
- Can be used as a high throughput screening tool for inhibitors of coronavirus proteases
CoV Protease Substrate-1 TF5 allows for efficient detection of coronavirus (CoV) proteases. This fluorescence resonance energy transfer (FRET)-based peptide substrate is cleaved by 3C-like proteases (3CLᵖʳᵒ), the CoV main protease involved in regulating the coronavirus replication complex. 3CLᵖʳᵒ is also a potential target for therapeutic development.
CoV Protease Substrate-1 TF5 has an excitation wavelength of 649 nm, an emission wavelength of 664 nm, and an extinction coefficient of 250,000 cm⁻¹ M⁻¹. It consists of a 14 amino acid sequence (KTSAVLQSGFRKME) that also contains the Tide Quencher™ 5 (TQ5; quencher) and Tide Fluor™ 5 (TF5; donor) fragments at the N- and C- termini, respectively. When the peptide substrate is intact, favorable energetic overlap of the TF5 excited state and the TQ5 absorption leads to efficient intramolecular fluorescence quenching of TF5 by the TQ5 fragment. Cleavage of the substrate by CoV proteases, however, dissociates the TF5 fragment from TQ5, restoring the fluorescence of the TF5 moiety that is directly correlated to the CoV protease activity.
Attention: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').