0219481110
0219481180
0219481190
ICNA0219481110EA
51.2
EUR
InStock
ICNA0219481110
ICNA0219481180
ICNA0219481190
X-Gal (Bromo-5-chloro-4-indolyl-3-ß-D-galactopyranoside) ≥98%, poudre blanche pour la biologie moléculaire
X-Gal (Bromo-5-chloro-4-indolyl-3-ß-D-galactopyranoside)
Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.
- Molecular Biology Reagent Grade
- Optical Rotation: (c=1, DMF:H2O 1:1): -62.0 ° (Lit.)
- Soluble in 50 mg/mL, DMF : MeOH (1:1). Soluble in DMF (2% w/v - clear, colorless solution).
Storage temperature: -20 °C