10009542-50
10009542-100
10009542-250
CAYM10009542-50EA
76.2
EUR
InStock
CAYM10009542-50
CAYM10009542-100
CAYM10009542-250
Anhydrotetracycline hydrochloride ≥98%, crystallin
Anhydrotetracycline hydrochloride
Anhydrotetracycline (hydrochloride) is a powerful effector in both the tetracycline repressor (TetR) and reverse TetR (revTetR) systems, binding the Tet repressor 35-fold more strongly than Tet.
- Absorbance: λmax: 224, 272, 428 nm
The tetracycline repressor (TetR) is a transcriptional regulator which normally binds tightly to its palindromic tetO operator DNA, blocking gene expression. Tet causes the repressor to dissociate from the DNA, allowing transcription to occur. A novel reverse TetR (revTetR) requires tetracycline as a co-repressor to bind tetO and block transcription.
Anhydrotetracycline poorly binds the 30S ribosomal subunit, compared to Tet so it does not act as a general inhibitor of translation and is a poor antibiotic. The concentration of anhydrotetracycline that inhibits eukaryotic cell growth is more than a 1,000-fold above the dose that alters transcription through TetR.
Stability: ≥2 years
A crystalline solid