21820-1
21820-10
21820-5
CAYM21820-1EA
55.5
EUR
InStock
CAYM21820-1
CAYM21820-10
CAYM21820-5
4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulphonic acid dipotassium salt ≥98%, solide cristallin colorant fluorescent
4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulphonic acid dipotassium salt
bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation. Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.
- Emission 523 (free), 496 (bound) nm
- Excitation 390 (free), 484 (bound) nm
- λmax 222, 275, 395 nm
bis-ANS has an excitation maxima of 390 nm. It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices. It also potently inhibits microtubule assembly.