16181
16182
PIER16181EA
163
EUR
InStock
PIER16181
PIER16182
Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific
Tests
Tests enzymatiques
The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.
- Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
- Sensitive—measure bright blue Gaussia and red firefly luciferase activities in the same sample
- Fast—no quenching step required, unlike in traditional sequential dual assays
- Multiplex—capable of quantitating two cellular activities in the same sample(s)
Gaussia luciferase acts as an experimental reporter with constitutively active red firefly as a normalisation control. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.
Information de livraison: Kit includes Gaussia-Firefly dual assay buffer (5 ml), 1X Coelenterazine (5 µl), D-Luciferin, Lyophilised (3 mg) and 2X Cell Lysis Buffer (6 ml).
Attention: For research use only. Not for use in diagnostic procedures.