M-Clarity™ quality level = MQ200
84120-100G
84120-25G
84120-100GEA
127
EUR
InStock
84120-100G
84120-25G
Safranine O pour la microscopie, Sigma-Aldrich®
Safranine O
Safranin O, also known as basic red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalised positive charge, an alternation of aromatic and quinonoid structures, and diazotisable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.
- Safranin O is widely employed as a microbiological stain, especially as a counterstain in gram staining to distinguish gram-positive from gram-negative micro-organisms, and for staining bacterial spores
- It is used to demonstrate glycosaminoglycans in human and animal histological specimens and nuclei
- It is a component of Benda's polychrome stain
- It is a component of various polychromes and lignin stains for botanical staining applications
- It has also been applied for the demonstration of mast cells in cytospin preparations and staining surgical frozen sections
- It is a diagnostic assay manufacturing, hematology, histology, microbiology