100-0470
100-0472
100-0471
STMC100-0470EA
883
EUR
InStock
STMC100-0470
STMC100-0472
STMC100-0471
ArciTect™ Human CRISPR Optimisation Kits (APC, PE, FITC)
Tests
Tests cellulaires
Complete kit for optimisation of genome editing in human cells.
The ArciTect™ Human CRISPR Optimisation kit is a flow cytometry-based kit designed to enable rapid optimisation of genome editing in human cells. It can also be used as a positive control for experiments using the ArciTect™ CRISPR-Cas9 genome editing system in human cells. The kit comprises ArciTect™ Human B2M sgRNA, ArciTect™ Cas9 Nuclease, and a fluorophore-conjugated beta-2 microglobulin (B2M) antibody. ArciTect™ Cas9 Nuclease must first be complexed with ArciTect™ Human B2M sgRNA, followed by delivery into human cells and subsequent culture for 48 to 96 hours. Following culture, editing efficiency can be assessed quickly by collection and processing for flow cytometry using a fluorophore-conjugated B2M antibody.
This kit has been tested and validated for use with the ArciTect™ line of genome editing products. By targeting the B2M gene that encodes a ubiquitously expressed cell-surface protein, editing efficiency can be rapidly and quantitatively assessed using flow cytometry methods. This technique also enables additional evaluation of editing success such as viability monitoring and/or assessment of cell type-specific marker expression.
Attention: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').