Synonyms:
5-FOA, 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid, 5-Fluoro-2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylic acid, 5-Fluorouracil-4-carboxylic acid
0215421050
0215421083
0215421091
ICNA0215421050EA
236
EUR
InStock
ICNA0215421050
ICNA0215421083
ICNA0215421091
Acide 5-fluoroorotique ≥98%, poudre cristalline jaune pâle
Acide 5-fluoroorotique
FOA is a derivative of a pyrimidine precursor and is selectively toxic to yeast cells which synthesize orotidine-5’-phosphate decarboxylase. FOA has been used in a positive selection for uracil-requiring mutants(in the presence of large numbers of wild-type cells) lacking OMPdecase or OPRTase activities. This results in efficient strains for effective transformation systems.
5-Fluoroorotic acid is useful in the selection of orotidine-5′-phosphate decarboxylase mutants of Saccharomyces cerevisiae.It has been used as a selective agent in yeast molecular genetics.It has also been used in the positive selection for uracil auxotrophs of the sulphur-dependent thermophilic archaebacterium sulpholobus acidocaldarius.
Soluble in hot water or methanol; soluble in 1:1 NH4OH : H2O (50 mg/ml with gentle heating).