28-9006-54
28-9006-46
28-9006-53
28-9006-54EA
1019
EUR
InStock
28-9006-54
28-9006-46
28-9006-53
illustra™ Hot Start mix, Ready-To-Go™
Réactifs pour acides nucléiques
End-point PCR Enzymes and Kits
Les réactifs PCR préformulés et prédistribués, cryodéshydratés associent la polymérase d'ADN PuReTaq™ haute qualité et une protéine activatrice Hot Start, pour une meilleure spécificité et reproductibilité des amplifications PCR. Les billes Ready-To-Go™ sont fournies dans des tubes de 0,5 ml ou 0,2 ml qui sont compatibles avec la plupart des thermocycleurs.
- La protéine d'activation recombinante Hot Start emprisonne les amorces avant la PCR, réduisant ainsi efficacement la formation d'une amorce non spécifique ou d'un dimère d'amorce
- Cela limite le risque de contamination depuis un mammifère associé aux anticorps Hot Start
- L'activation rapide Hot Start préserve l'intégrité des échantillons en évitant un traitement thermique de pré-cyclage prolongé
- Température ambiante stable sur le long terme: plus de perte de place dans le congélateur; faible consommation énergétique pour le transport et la conservation
- Adapté au PCR en temps réel
Kit includes room-temperature stable beads containing 1.75 units of recombinant PuReTaq DNA polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction buffer. When a bead is reconstituted to a 25 μl final volume, the concentration of each dNTP is 200 μM and the concetration of MgCl₂ is 1.5 mM.
The additional reagents required are water, primers, and template DNA. MgCl₂ can be added, allowing users to customize this reagent to their specific needs. The beads are provided predispensed into either 0.2 or 0.5ml PCR tubes compatible with most thermal cyclers. The 0.2 ml tubes come assembed in a 96-well (8×12) plate format that allows individual strips of eight tubes to be easily removed. This flexibility allows use of the entire 96-well plate, strips of eight, or individual 0.2 ml tubes. Avoids the risk of contamination from a mammalian source associated with hot start antibodies.
Quick hot start activation to preserve sample integrity by avoiding extensive precycling heat treatment.
Long-term ambient-temperature stable: No freezer space required - Less energy consumption for shipping and storage.
This Ready-To-Go format provides reproducible and reliable performance for demanding PCR applications in which high specificity and sensitivity are essential to success.
The system is based on a novel hot start method that uses a hot start activator protein to sequester primers prior to PCR, thereby making them unavailable for nonspecific priming during the reaction preparation. This method requires only a quick 2 min initial PCR activation step. In contrast, other available hot start PCR kits that are based on chemical inactivation require prolonged incubation at high temperatures, which may damage the DNA template by heat-induced depurination. The net result from using Hot Start Mix RTG is a more robust and reliable amplification of the target amplicons with increased yield when compared with conventional hot start master mix products. Furthermore, the mix does not contain Taq antibody and this eliminates the risk of mammalian-source contamination.