Ace+Method+Development+Kits
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Fournisseur:
OMEGA BIO-TEK
Description:
Le kit E.Z.N.A.® Plant RNA offre une méthode pratique et rapide pour l'isolation de l'ARN total à partir d'un grand nombre d'échantillons végétaux. Ce kit fournit une colonne d'homogénéisation pour la filtration et l'homogénéisation des lysats cellulaires végétaux visqueux par centrifugation en association avec la colonne de centrifugation d'ARN HiBind® pour la purification d'ARN. Tous les contaminants, y compris les polysaccharides et les composés phénoliques, sont efficacement supprimés. L'ARN purifié peut être utilisé pour la plupart des applications en aval telles que la RT-PCR, l'analyse par transfert d'ARN, la technique du DD, et la sélection d'ARN poly(A)+.
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Numéro de catalogue:
(BNUM0950-50)
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
UOM:
1 * 50 µl
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Fournisseur:
Biotium
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Fournisseur:
OMEGA BIO-TEK
Description:
Le kit E.Z.N.A.® Viral RNA est conçu pour l'isolement de l'ARN viral à partir de liquides exempts de cellules, tels que le plasma, le sérum, l'urine et le surnageant de culture cellulaire. Cette procédure élimine totalement les contaminants et les inhibiteurs d'enzymes, ce qui le rend l'isolement d'ARN viral rapide, pratique et fiable. Le kit est également adapté à l'isolement de l'ARN total à partir de cellules en culture, de tissus et de bactéries. L'ARN purifié à l'aide de la méthode E.Z.N.A.® Viral RNA est prêt à l'emploi pour toutes les applications en aval telles que la RT-PCR.
Numéro de catalogue:
(TNB-6600-KIT)
Fournisseur:
Tonbo Biosciences
Description:
The fragmentation of genomic DNA by cellular nucleases during the later stages of apoptosis is also one of the most easily measured features of apoptotic cells. Nuclease activity generates DNA fragments ranging from ~300 bp to 50 bp in length, resulting in a typical DNA ‘laddering’ appearance when analyzed by agarose gel electrophoresis. These fragments have exposed 3’-hydroxyl (OH) ends which can be labeled with bromolated deoxyuridine triphosphates (Br-dUTP). An enzyme, terminal deoxynucleotidyl transferase (TdT), is used to catalyze the template-independent addition of Br-dUTP to the 3’-OH ends of double or single stranded DNA. This method is often called TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) or end labeling. Sites where the Br-dUTP is incorporated can then be detected with an antibody specific to BrdU. With the APO-BrdUTM Kit cells are first labeled with Br-dUTP, and then sites of incorporation are detected through staining with a FITC anti-BrdU antibody. Samples can then be analyzed via flow cytometry. Samples that are apoptotic will stain brightly with the anti-BrdU antibody due to the substantial number of exposed 3’-OH sites, while cells that are non-apoptotic will not have incorporated significant amounts of Br-dUTP and will stain dimly. The APO-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.
UOM:
1 * 50 Tests
Fournisseur:
OMEGA BIO-TEK
Description:
E.Z.N.A.® Plasmid Midi Kit propose une méthode fiable et novatrice pour isoler jusqu'à 200 µg d'ADN plasmidique en moins de 60 minutes. Ce kit associe la technologie de membrane HiBind® à la cohérence durable de la lyse alcaline SDS de cellules bactériennes afin d'offrir de l'ADN plasmidique de haute qualité. Les colonnes HiBind® DNA midi exemptes de DNase/RNase fonctionnent en éliminant les processus fastidieux d'extractions de phénol/chloroforme et de précipités d'alcool. La purification plasmidique faisant appel au E.Z.N.A.® Plasmid Midi Kit suit une procédure simple de liaison, lavage et d'élution permettant le traitement de plusieurs échantillons. Bien que les rendements varient en fonction du nombre de copies d'ADN, la souche <i>E. coli</i>, et les conditions de croissance, 50 ml d'une culture sur une nuit dans un milieu LB produit généralement 200 µg de nombre élevé de copies d'ADN plasmidique. Lorsque vous travaillez avec un faible nombre de copies de plasmides, le volume de départ de la culture peut être augmenté jusqu'à 100 ml. Un ADN de haute qualité est prêt pour une utilisation immédiate dans les applications de biologie moléculaire, telles que le séquençage automatisé fluorescent, la restriction de la digestion enzymatique, le dépistage et la transfection.
Fournisseur:
OMEGA BIO-TEK
Description:
Le kit E-Z 96® Total RNA est conçu pour l'isolement de l'ARN cellulaire total provenant d'au plus 5×10⁶ cellules en culture ou tissus mous. Ce kit peut traiter des échantillons simples ou multiples en moins de 60 minutes. Comme il utilise la technologie des plaques de silice HiBind®, les extractions au phénol/chloroforme, l'ultracentrifugation en gradient de CsCl et la précipitation à l'isopropanol ou LiCl sont superflues. Les échantillons sont lysés dans un tampon de lyse dénaturant qui désactive les RNases. Les conditions de liaison sont ajustées et le lysat est transféré vers une plaque de 96 puits HiBind® RNA où l'ARN est purifié par trois étapes de lavage. L'ARN de grande qualité est élué dans de l'eau exempte de RNase. L'ARN purifié à l'aide de la méthode E-Z 96® Total RNA est prêt à l'emploi pour des applications telles que la RT-PCR, la qPCR, le DD, les microéchantillons, ainsi que pour d'autres applications en aval.
Numéro de catalogue:
(D2492-03)
Fournisseur:
OMEGA BIO-TEK
Description:
Le kit E.Z.N.A.® Blood DNA Maxi est spécifiquement conçu pour l'isolation à grande échelle d'ADN génomique. Ce kit permet une purification rapide d'ADN génomique à partir d'échantillons de 10 ml de sang total. Des sources d'échantillons incluent du sang total frais et congelé traité avec des anticoagulants courants tels que le citrate, l'EDTA et l'héparine. Il est également possible d'utiliser des échantillons de plasma, sérum, couche leuco-plaquettaire, moelle osseuse, lymphocytes, plaquettes et liquides organiques. Les extractions au phénol/chloroforme, ainsi que les étapes fastidieuses telles que la précipitation à l'isopropanol, ont été éliminées. L'ADN purifié à l'aide de la méthode E.Z.N.A.® Blood DNA Maxi est exempt de contaminants et d'inhibiteurs d'enzyme, ce qui permet de l'utiliser dans la plupart des applications en aval, par exemple PCR, transfert Southern et digestion par des enzymes de restriction d'ADN total de haute qualité.
UOM:
1 * 50 Tests
Appel de prix
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